Kits and Components
Accurately detecting rare antigen-specific T and/or B cells has long been a significant challenge in immune monitoring. For more than two decades, CTL has been at the forefront of this field with its advanced ImmunoSpot® platform.
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ImmunoSpot® test kits are carefully optimized to deliver maximum sensitivity for detecting and analyzing the secretory footprint of individual T and B cells, as well as to enable clear assessment of co-expression patterns.
Using a 4-color ImmunoSpot® kit, CD8+ T cell subpopulations can be simultaneously identified by measuring IFN-γ, TNF-α, GzB, and IL-2. (Learn more). See kit.
For CD4+ T cell subpopulations, Th1, Th2, Th17, and Th21 can be characterized based on their secretion of IFN-γ, IL-4, IL-17, and IL-21, respectively. However, multiplex analysis of these subsets must take into account their differential activation kinetics. (Learn more). CTL provides PBMC/antigen pairs that show Th1, Th2, Th17, and Th21 reactivity, available as positive controls and reference samples (please inquire).
Multiplexed detection of B cells secreting various immunoglobulin classes and/or IgG subclasses is also possible with up to 4-color kits. Additionally, we offer support in developing antigen-specific B cell assays by formulating the most appropriate coating conditions (please inquire).
NK cell and ADCC measurements have never been more straightforward and efficient (learn more).
All our kits are developed and standardized to eliminate variability and reduce subjectivity in performance. Our T cell kits include serum-free assay medium, removing the need for blocking steps and eliminating batch-to-batch variability common with FBS-containing media in functional assays.
Each ImmunoSpot® ELISPOT Kit includes all required buffers and washing solutions, and comes with our serum-free CTL-Test™ Medium, specially formulated for low background and high antigen-specific signal in T cell ImmunoSpot® assays. We also offer serum-free media for freezing and thawing cryopreserved PBMC.
To support your assay development, CTL provides peripheral blood mononuclear cells (PBMC) with well-defined characteristics. These serve as reliable positive controls and reference samples and include PBMCs with Th1, Th2, Th17 cytokine signatures, or antibody-secreting cell (ASC) reactivity against specific antigens (please inquire to learn more).
If you do not yet have an ELISPOT/FluoroSpot Reader, our ScAnalysis™ Service is a great way to get started.
We are here to support your immune monitoring efforts — feel free to contact us anytime.
ImmunoSpot® Kit Categories
T Cell Kits
It include capture and detection antibodies, enzymatic or fluorescent detection reagents, diluent buffers, and PVDF membrane plates—everything needed for reliable T cell assay performance
B Cell Kits
B Cell Kits are supplied with capture and detection antibodies, enzymatic or fluorescent detection reagents, diluent buffers, and PVDF membrane plates, providing all essential components for accurate B cell assay execution.
Human NK-TVA Kits
Human NK-TVA Kits enable reliable detection of fluoresce-labeled target cells’ lysis at single-cell resolution.
Cell Counting Kits & Component
It allows for live/dead counting of PBMC, selectively staining live, dead, and apoptotic cells with high accuracy.
Ferret Antibodies
Ferret Antibodies are designed to recognize specific heavy or light chains on the ferret B cell receptor (BCR). They are suitable for use in Western Blot, ELISA, Flow Cytometry, ELISPOT, and FluoroSpot applications.