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Neutralization Assays and Colony Counting

Immune monitoring often requires assessing the effectiveness of serum antibodies in neutralizing viruses, bacteria, or toxins. ImmunoSpot® Readers fully support these high-throughput measurements, including colony counting across various well formats — from 384-well plates to Petri dishes.

Learn more about leveraging ImmunoSpot® Readers for high-throughput neutralization assays.

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Here’s an overview of viral assays quantified by ImmunoSpot® Readers:

  • Viral titer

  • Plaque Reduction Neutralization Assay (PRNT)

  • Virus/serum neutralization

  • Viral immune-colorimetric assay (ICA)

  • Focus forming assay

  • Intracellular inclusion assay

  • Plaque Forming Unit / Focus-Forming Unit (PFU/FFU) assay

  • Cytopathic Effect (CPE)

For examples of ImmunoSpot® Readers used in SARS-CoV-2 assays, follow this link.
For a multi-color reference applying ImmunoSpot® Readers in viral studies, see J. Immunol., 2015, 195: 3490.

ImmunoSpot® Readers also excel in high-throughput bacterial colony counting across formats — from 384-well plates to Petri dishes and square agar slabs.
Commonly supported assays include:

  • Serum Bactericidal Assays (SBA)

  • Opsono-Phagocytotic Assays (OPK)

For a key bacterial colony counting reference, see J. Immunol. Methods, 2004, 292: 187.

They are also highly effective for high-throughput yeast colony counting in formats ranging from 384-well plates to Petri dishes. Applications include:

  • Humanized yeast models

  • Proteomics (Y2H)

  • Antibiotic testing

  • Toxicology

For a reference in this context, see Cell Death & Disease, 2011, 2:188.

High throughput is critical in colony counting. Optimized ImmunoSpot® Readers can count a 384-well plate in under a minute — processing up to 400 plates in a single run.

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